GeneDn: for high-level expression design of heterologous genes in a prokaryotic system
نویسندگان
چکیده
منابع مشابه
prokaryotic high-level expression system in producing adhesin recombinant protein e of nontypeable haemophilus influenzae
conclusions the pe gene was successfully cloned and confirmed by sequencing. finally, pe was obtained with high concentration. due to high homology and similarity among the pe gene from nthi atcc 49766 and other nthi strains in genbank, we believe that the protein is a universal antigen to be used as a vaccine design candidate and further studies to evaluate its immunogenicity is underway. mate...
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Over Expression of Influenza Virus M2 Protein in Prokaryotic System
Background and Aims: Influenza A virus of Orthomyxoviridae family is able to create pandemic influenza. Vaccination is the most effective way to prevent influenza virus infection. Matrix protein 2 (M2) is a homotetramer ion channel with 97 amino acids length and highly conserved among influenza viruses and is considered for development of a universal influenza vaccine. Materials and Methods: We...
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LCR/MEL: a versatile system for high-level expression of heterologous proteins in erythroid cells.
We have used the human globin locus control region (LCR) to assemble an expression system capable of high-level, integration position-independent expression of heterologous genes and cDNAs in murine erythroleukaemia (MEL) cells. The cDNAs are inserted between the human beta-globin promoter and the second intron of the human beta-globin gene, and this expression cassette is then placed downstrea...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 1998
ISSN: 1367-4803,1460-2059
DOI: 10.1093/bioinformatics/14.10.884